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对78例下呼吸道感染患儿的鼻咽分泌物标本同时采用间接免疫荧光法(IF)、碱性磷酸酶抗碱性磷酸酶桥联酶法(APAAP)及病毒分离,进行呼吸道合胞毒检测,三者的总检出率分别为41％,46％和31％。IF与APAAP总检出率之间无显著差别,二者的敏感度与特异度相近,敏感度分别为88％和92％,特异度分别为93％和91％。后者因避免了IF需要较昂贵的荧光显微镜及标本不易保存的缺点,而适于在我国基层医院使用,值得推广。
Nasopharyngeal secretions samples from 78 children with lower respiratory tract infection were detected by indirect immunofluorescence assay (IFA), alkaline phosphatase-resistant alkaline phosphatase (APAAP) and virus isolation for respiratory syncytial virus , The total detection rate of the three were 41%, 46% and 31%. There was no significant difference between the detection rates of IF and APAAP. The sensitivity and specificity of IFA and APAAP were similar, with the sensitivity of 88% and 92% respectively, and the specificity of 93% and 91% respectively. The latter due to the need to avoid the IF more expensive fluorescent microscope and the shortcomings of the specimen is not easy to save, but suitable for use in primary hospitals in our country, it is worth promoting.