论文部分内容阅读
以硝酸异山梨酯为内标，乙酸乙酯为萃取溶剂，建立了５－单硝酸异山梨酯血浆样品经液－液萃取分离后，毛细管气相色谱电子捕获检测的灵敏、准确的定量分析方法。对血浆中５－单硝酸异山梨酯测定的最低检出量为２ｎｇ／ｍｌ，血药浓度在１０～６００ｎｇ／ｍｌ范围内色谱响应线性良好。并用于５－单硝酸异山梨酯药动学的研究。
An isosorbide dinitrate was used as the internal standard and ethyl acetate was used as the extraction solvent. A sensitive and accurate quantitative analysis method was established for the determination of isosorbide 5-mononitrate plasma samples by liquid-liquid extraction and capillary gas chromatography electron capture. The detection limit of isosorbide 5-mononitrate in plasma was 2ng / ml. The linearity of chromatographic response was good in the range of 10 ~ 600ng / ml. And used for 5-mononitrate isosorbide pharmacokinetic study.