论文部分内容阅读
目的为了避免免疫组化染色试验中抗体溢出组织,使抗原组织与抗体得不到充分的孵育及反应。因此,必需使用免疫组化笔在切片组织周围画圈避免抗体流出。方法一支废旧的免疫组化液体石蜡笔,用小瓶倒入2 ml树胶,加入适量二甲苯稀释树胶,用此笔蘸稀释后的树胶在玻片组织周围画圈。结果再利用自制免疫组化笔的隔水率达90%,同美国进口免疫组化笔及国产笔其隔水率效果相同,增强了免疫组化阳性细胞的表达效果。结论废旧自制免疫组化笔完全可以代替任何国产、进口免疫组化笔,且价格低廉。
Purpose In order to avoid the overflow of the antibody in the immunohistochemical staining test, the antigen tissue and the antibody can not be fully incubated and reacted. Therefore, it is necessary to use an immunohistochemical pen to circle the sectioned tissue to prevent the antibody from flowing out. Method A waste immunohistochemistry liquid crayon, pour 2 ml of vial into a vial, dilute the gum with appropriate amount of xylene, and dip the diluted gum around the tissue of the slide. Results Reuse of self-made immunohistochemical pen water barrier rate of 90%, with the United States imported immunohistochemical pen and domestic pen the same effect of water, enhance the expression of immunohistochemical positive cells. Conclusion Waste homemade immunohistochemical pen can replace any domestic, imported immunohistochemical pen, and the price is low.