根据绒毛取样(CVS)后,诊断染色体异常的经验,加强了对应用长期细胞培养支持短期染色体制备的观点(短期=直接制片及24小时培养制片)。来自滋养外胚层的朗罕氏层快速分裂的细胞,经醋酸处理后用于短期法分析,而来自中胚层的绒毛中心细胞,则是长期培养的主要基础。前者制备的染色体其质量常属一般,而后者则可提供获得最高质量染色体的方法。但是,用于诊断的目的,长期绒毛细胞培养至少有三个不利条件。第一,母体细胞污染的危险较高。第二,长期组织培养易 The experience of diagnosing chromosomal abnormalities following villus sampling (CVS) reinforces the notion that short-term chromosomal preparation is supported by long-term cell culture (short-term = direct and 24-hour culture). Cells rapidly dividing from the raxane layer that nourish the ectoderm were treated with acetic acid for short-term analysis, while villus central cells from the mesoderm were the main basis for long-term culture. Chromosomes produced by the former are generally of the same quality, while the latter provide the method of obtaining the highest quality chromosomes. However, for the purpose of diagnosis, long-term villus cell culture has at least three disadvantages. First, there is a higher risk of maternal cell contamination. Second, long-term tissue culture easy