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目的了解上海市浦东新区副溶血弧菌病原学与分子流行病学特征。方法运用玻片凝集法对505株副溶血弧菌菌株进行血清学分型,运用K-B纸片法对菌株进行12种抗生素药敏试验,同时对菌株基因组DNA经限制性内切酶NotⅠ酶切后进行脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)分子分型,利用BioNumericsVersion 6.64分析软件对图谱进行聚类分析,初步建立PFGE分子分型数据库。结果 505株菌株中有117株血清型未能分型,O3∶K6为患者分离株中的优势血清型,其中69.14%(56/81)的食物中毒分离株和61.87%(185/299)的散发病例分离株血清型为O3∶K6型;监测食品分离株血清型分布呈现多样性,无优势菌株。99.41%(502/505)菌株对氨苄西林耐药。505株菌株共获得221个不同的PFGE带型,有26株PFGE未能分型。监测食品分离株的PFGE呈现遗传多样性,无优势带型,并且与散发及食物中毒患者分离株的PFGE带型不同。食物中毒与散发病例分离株的优势带型相同。耐2种或以上抗生素的菌株与其他菌株的PFGE型不相同,相同PFGE带型内的菌株血清型相同,不同时间、不同腹泻监测点之间存在完全相同PFGE条带。结论浦东新区未出现多重耐药菌株,存在多起疑似聚集性病例事件,但与监测食品分离株的遗传谱系关系较远。
Objective To understand the etiological and molecular epidemiological characteristics of Vibrio parahaemolyticus in Pudong New Area of Shanghai. Methods Serological typing of 505 strains of Vibrio parahaemolyticus was carried out by slide agglutination method. The drug susceptibility tests of 12 antibiotics strains were carried out by KB paper method. The genomic DNA of the strains were digested with restriction endonuclease Not Ⅰ Pulsed field gel electrophoresis (PFGE) molecular typing, using BioNumericsVersion 6.64 analysis software to cluster analysis of the map, the initial establishment of PFGE molecular typing database. Results Among the 505 isolates, 117 serotypes failed to be typed. O3: K6 was the predominant serotype in the isolates of the patients. Among them, 69.14% (56/81) of food poisoning isolates and 61.87% (185/299) The serotypes of the isolates were distributed as O3: K6. The distribution of serotypes in foodstuff isolates was diversified without predominant strains. 99.41% (502/505) strains were resistant to ampicillin. A total of 221 strains of PFGE were obtained from 505 strains, and 26 strains of PFGE could not be typed. The PFGE of foodstuff isolates showed genetic diversity without dominant bands and differed from the PFGE patterns of isolates from sporadic and food poisoning patients. Food poisoning and sporadic cases of isolates with the same dominant band. The strains with two or more antibiotics were different from other strains in PFGE type. The strains in the same PFGE band had the same serotype. Identical PFGE bands existed between different diarrhea monitoring points at different times. Conclusion There was no multidrug-resistant strains in Pudong New Area, and there were many cases of suspected cluster events, but the genetic lineages were far away from monitoring the genetic lineages of food isolates.