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目的:根据不同品种陈皮中3种黄酮类成分橙皮苷、川陈皮素和橘皮素的含量比值,鉴别广陈皮道地性。方法:采用Acquity UPLC BEH C_(18)(2.1 mm×50 mm,1.7μm)色谱柱,以乙腈-0.1%磷酸水为流动相进行梯度洗脱,流速0.42 mL·min~(-1),柱温30℃,检测波长283 nm,测定时间12min;运用UPLC对92批11种不同来源的陈皮药材进行测定,不同品种陈皮中橙皮苷、川陈皮素和橘皮素含量比值不相同,并对其进行系统聚类分析和主成分分析。结果:广陈皮中橙皮苷、川陈皮素和橘皮素含量分别(34.72±1.74)mg·g~(-1)、(3.36±1.62)mg·g~(-1)、(1.83±0.47)mg·g~(-1),含量比值为19:2:1。系统聚类分析和主成分分析将不同品种陈皮分成4类,能准确把茶枝柑归为一类。其中红橘与茶枝柑的3种黄酮类成分含量比值相近。结论:该法为广陈皮的道地性鉴别提出新方法。
OBJECTIVE: According to the content ratio of hesperidin, nobiletin and hesperidin in three varieties of dried tangerine peel, the characters of Guangcai authenticity were identified. Methods: The mobile phase was eluted with acetonitrile (0.1% phosphoric acid) with a mobile phase of 0.42 mL · min -1 using Acquity UPLC BEH C 18 (2.1 mm × 50 mm, 1.7 μm) Temperature 30 ℃, detection wavelength 283 nm, determination time 12 min; UPLC was used to determine the content of hesperidin, nobiletin and hesperidin in 92 batches of 11 different types of dried tangerine peel from different sources, It conducts systematic cluster analysis and principal component analysis. Results: The contents of hesperidin, nobiletin and hesperidin in Guangcai skin were (34.72 ± 1.74) mg · g -1, (3.36 ± 1.62) mg · g -1, (1.83 ± 0.47 ) mg · g -1, the content ratio is 19: 2: 1. System cluster analysis and principal component analysis of different varieties of tangerine peel is divided into four categories, can be accurately classified as Citrullus versicolor. Among them, the ratio of the content of three flavonoids in red tangerine and tea tree citrus is similar. Conclusion: This method proposed a new method for the authenticity identification of broad-leaved citrus peel.