目的评价鞘内注射雷帕霉素对CCI神经病理性痛大鼠的痛阈及脊髓背角胶质细胞表达的影响。方法健康雄性SD大鼠30只随机分为6组:1CCI组:CCI术后14天处死;2正常对照组:不做任何处理;3前对照剂组:鞘内置管3天后行CCI术,术后4小时后鞘内给同体积生理盐水,连给3天;4前给药组:鞘内置管3天后行CCI术,术后4小时鞘内给雷帕霉素溶液,连给3天;5后对照剂组:鞘内置管3天后行CCI术,术后7天鞘内给同体积生理盐水,连给3天;6后给药组:鞘内置管3天后行CCI术,术后7天鞘内给雷帕霉素溶液,连给3天。各组于CCI术前1天和术后第2、4、6、8、10、12、14天测机械痛阈和热痛阈。术后14天测痛后用多聚甲醛灌注大鼠,取L4~5脊髓,免疫组化染色,星形胶质细胞标记蛋白(GFAP)检测星形胶质细胞表达变化,并定量分析。结果与对照组相比,CCI手术组热痛阈和机械痛阈从CCI手术后第4天开始下降(P<0.05);前后给药对照剂组与CCI组相比,差别无统计学意义(P>0.05)。前给药组痛阈从CCI手术后第4天开始上升并持续至手术后第14天,与CCI组相比,差别有统计学意义(P<0.05)。与CCI组相比,后给药组痛阈从CCI第8天开始上升并持续至手术后第14天,差别有统计学意义(P<0.05)。与正常对照组比较,CCI组、前、后对照剂组手术侧脊髓背角GFAP染色阳性区平均光密度与阳性面积均有增加,差别有统计学意义(P<0.05)。前、后给药组手术侧GFAP染色阳性区平均光密度与阳性面积与CCI组比较,均有明显降低,差别有统计学意义(P<0.05)。结论鞘内注射雷帕霉素可缓解大鼠神经病理性痛,并抑制脊髓背角胶质细胞的激活。 Objective To evaluate the effect of intrathecal rapamycin on the pain threshold and the expression of glial cells in spinal dorsal horn of CCI rats with neuropathic pain. Methods Thirty healthy male Sprague-Dawley rats were randomly divided into 6 groups: 1CCI group: sacrificed 14 days after CCI; 2 normal control group: without any treatment; 3 before control group: CCI after 3 days intrathecal catheterization 4 hours after intrathecal administration of the same volume of saline, even for 3 days; 4 before treatment group: intrathecal catheterization after 3 days CCI surgery, 4 hours after intrathecal rapamycin solution, even for 3 days; 5 after the control group: intrathecal catheter after 3 days of CCI surgery, 7 days after intrathecal administration of the same volume of saline, even for 3 days; 6 after the administration group: intrathecal catheter 3 days after CCI surgery, 7 Days to intrathecal rapamycin solution, even for 3 days. The mechanical pain threshold and thermal pain threshold of each group were measured at 1 day before CCI and days 2,4,6,8,10,12,14 after operation. Fourteen days after surgery, rats were anesthetized with paraformaldehyde, and the spinal cord of L4-5 was taken for immunohistochemical staining and astrocyte marker protein (GFAP) to detect the changes of astrocytes. Quantitative analysis was performed. Results Compared with the control group, the pain thresholds and mechanical pain thresholds in CCI group decreased from the 4th day after CCI (P <0.05), and there was no significant difference between CCI group and CCI group P> 0.05). The pain threshold of the former medication group increased from the 4th day after the CCI operation and continued to the 14th day after the operation. The difference was statistically significant (P <0.05) compared with the CCI group. Compared with the CCI group, the pain threshold of the post-administration group increased from the 8th day of CCI and continued to the 14th day after the operation, the difference was statistically significant (P <0.05). Compared with the normal control group, the mean optical density and the positive area of ​​GFAP positive area in spinal dorsal horn of CCI group, anterior and posterior contrast group were increased, the difference was statistically significant (P <0.05). Compared with CCI group, the average optical density and the positive area of ​​GFAP positive area in the operation group before and after administration were significantly decreased (P <0.05). Conclusion Intrathecal injection of rapamycin can relieve the neuropathic pain in rats and inhibit the activation of glial cells in the dorsal horn of spinal cord.