氯霉素滴眼液为医院的常用制剂,常用0.03～0.06%对羟基苯甲酸乙酯作抑菌剂,当用紫外分光光度法测定时,由于后者干挠,结果偏高甚多。为了消除干挠,刘耀华用二波长吸收系数解联立方程计算法,洪东等用双波长法测定。本文用正交函数分光光度法测定氯霉素的含量,测得回 Chloramphenicol eye drops for the hospital’s commonly used preparations, commonly used 0.03 to 0.06% of ethylparaben as a bacteriostatic agent, when measured by UV spectrophotometry, due to the latter dry scratch, the result is much higher. In order to eliminate interference, Liu Yaohua with two wavelength absorption coefficient solution of simultaneous equations calculation method, Hong et al by dual wavelength method. In this paper, the orthogonal function spectrophotometric determination of chloramphenicol content, measured back