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目的探讨KLK14蛋白宫颈癌中的表达及与临床病理关系。方法应用免疫组织化学法SP检测10例正常宫颈14例慢性宫颈炎48宫颈癌组织中KLK14蛋白的表达。应用半定量逆转录聚合酶链反应法RT-PCR检测25例宫颈鳞癌及10例正常宫颈组织中KLK14mRNA的表达。结果 KLK14蛋白在48例宫颈癌中阳性率72.9%,正常宫颈20%,慢性宫颈炎14.3%,三者相比差异有统计学意义,KLK14蛋白阳性率与肿瘤临床分期,病理学相关(P<0.05),与年龄组织学分级淋巴转移无关(P>0.05)。KLK14mRNA在宫颈癌相对表达量高于正常宫颈组织。结论 KLK14表达参与宫颈癌的发生发展。
Objective To investigate the expression of KLK14 protein in cervical cancer and its relationship with clinicopathology. Methods Immunohistochemical SP method was used to detect the expression of KLK14 protein in 10 cases of cervical cancer tissues from 14 cases of chronic cervicitis with chronic cervicitis. The expression of KLK14 mRNA in 25 cases of cervical squamous cell carcinoma and 10 cases of normal cervical tissue was detected by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Results The positive rate of KLK14 protein in 48 cases of cervical cancer was 72.9%, 20% of normal cervix and 14.3% of chronic cervicitis. The positive rate of KLK14 protein was correlated with the clinical stage and pathology (P < 0.05), but not with histological grade lymph node metastasis (P> 0.05). The relative expression of KLK14 mRNA in cervical cancer is higher than that in normal cervical tissue. Conclusion The expression of KLK14 is involved in the development of cervical cancer.