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研究以树龄3200年的香竹箐茶树王为基础,应用27对引物对包括香竹箐茶树王在内的22份古茶树资源、44份野生茶树资源和18份栽培品种茶树资源进行了遗传多样性和亲缘关系的分析。结果表明,27对引物共扩增出190个基因型,91个等位基因,Shannon平均值为0.92。观测杂合度平均值0.52,期望杂合度平均值0.54,均大于0.50。PIC值在0.10~0.73,平均0.54,大于0.50。说明供试材料遗传差异大。香竹箐茶树王与古茶树、野生茶树和栽培型茶树中遗传相似性系数低于0.50的资源在每个群体分别占9.1%、29.6%和44.4%,说明香竹箐茶树王与野生茶树和栽培型茶树品种之间的遗传关系较远。聚类分析表明,84份材料按亲缘关系和地理来源分成了17个组,亲缘关系树状图在分子水平上显示了香竹箐茶树王与其他供试茶树资源之间的亲缘关系,为今后对香竹箐茶树王等宝贵茶树资源的保护、研究和开发提供有效的理论依据。
Based on 3200-year-old fragrant bamboo tea tree king, based on 27 pairs of primers, including 22 ancient tea tree resources, including 44 species of wild tea tree resources and 18 varieties of tea tree resources, Sexual and kinship analysis. The results showed that a total of 190 genotypes and 91 alleles were amplified by 27 pairs of primers. The Shannon average was 0.92. The average observed heterozygosity was 0.52 and the average expected heterozygosity was 0.54, both of which were greater than 0.50. PIC values ranged from 0.10 to 0.73 with an average of 0.54 and greater than 0.50. Explain the test material genetic differences. The average genetic similarity coefficient of Camellia sinensis and ancient tea tree, wild tea tree and cultivated tea tree were less than 0.50, accounting for 9.1%, 29.6% and 44.4% of the total in each group, indicating that the tea plant king and wild tea tree The genetic relationship between cultivated tea varieties is far. Cluster analysis showed that 84 materials were divided into 17 groups according to their genetic relationship and geographical origin. The phylogenetic tree showed the genetic relationship between the tea plant king and other tea resources at the molecular level. On the fragrant bamboo 箐 tea tree and other precious tea tree resources protection, research and development to provide an effective theoretical basis.