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目的研究避水应激引起内脏痛觉敏感性的变化及特异性辣椒素受体(VR1)拮抗剂辣椒平与非特异性VR1拮抗剂钌红对避水应激引起内脏痛觉过敏的治疗作用。方法将6周龄雄性Wistar大鼠54只分为无应激对照组(Neg组,n=18)、生理盐水对照组(NS组,n=12)、辣椒平治疗组(CZP组,n=12)、钌红治疗组(RR组,n=12),Neg组又分为三个小组,分别为直肠内注入生理盐水组、直肠内注入辣椒素组、直肠扩张组,NS组、CZP组和RR组只分为直肠内注入辣椒素组和直肠扩张组两小组,每小组均为6只动物。给予避水应激,每天1h连续10d。第11天Neg组与NS组腹腔注射溶媒,CZP组腹腔注射辣椒平,RR组腹腔注射钌红,30min后每小组6只动物直肠内注入生理盐水或辣椒素,进行疼痛评分,或给予结直肠扩张(CRD)刺激,进行腹直肌肌电记录。结果避水应激引起动物对直肠注射辣椒素敏感性增加,腹腔注射辣椒平与钌红均能抑制注射辣椒素引起的疼痛反应;避水应激也引起动物CRD刺激后腹直肌肌电活动增加,腹腔注射辣椒平与钌红均能降低肌电活动的增加幅度,而钌红则完全抑制肌电活动的增加。结论辣椒素受体拮抗剂可抑制避水应激引起的内脏痛觉过敏,辣椒素受体参与内脏痛觉过敏的发生过程。
Objective To study the changes of visceral hyperalgesia induced by water stress and the therapeutic effect of the specific and non-specific VR1 antagonist ruthenium on the visceral hyperalgesia induced by water stress. Methods Sixty-four male Wistar rats of 6 weeks old were divided into three groups: control group (Neg, n = 18), saline control group (NS group, n = 12) 12), ruthenium red group (RR group, n = 12). Neg group was divided into three subgroups: normal saline group injected into rectum, capsaicin group, rectum expansion group, NS group and CZP group And RR group were only divided into two groups rectal injection of capsaicin group and rectal distention group, each group is 6 animals. Given to avoid water stress, 1h per day for 10d. On the eleventh day, the mice in the Neg and NS groups were intraperitoneally injected with vehicle. The CZP group was intraperitoneally injected with chilly peppers. The rats in the RR group were intraperitoneally injected with ruthenium red. After 30 minutes, 6 animals in each group were injected with normal saline or capsaicin into the rectum for pain scores or colorectal Dilation (CRD) stimulation, electromyographic recording of the rectus abdominis. Results Avoid water stress caused the animals to increase the sensitivity to rectal injection of capsaicin, intraperitoneal injection of peppers and ruthenium red can inhibit the injection of capsaicin-induced pain response; water-avoidance stress also caused animal CRD stimulation of the rectus abdominis muscle myoelectric activity Increase, intraperitoneal injection of peppers and ruthenium red can reduce the increase in myoelectric activity, while ruthenium red completely inhibited the increase in myoelectric activity. Conclusion Capsaicin receptor antagonist can inhibit visceral hyperalgesia induced by water stress and capsaicin receptor participates in the process of visceral hyperalgesia.