Na~+-K~+-腺苷三磷酸酶(Na~+-K~+-ATP酶),在肾重吸收Na~+的过程中起着重要的作用,具有维持细胞内Na~+与细胞外K~+按比例进行交换的作用,它在肾中分布不均,研究该酶在肾小管中分布情况对了解肾Na~+重吸收功能及病理变化有一定的意义.本实验将Mayakara等人的柠檬酸铅法,稍加改变,应用对硝基苯酚磷酸(P--nitrophenylphosphate Na salt P-NPP钠盐)作底物,可获同样效果,灵敏度高,结果可靠. Na ~ + -K ~ + adenosine triphosphatase (Na ~ + -K ~ + -ATPase) plays an important role in renal reabsorption of Na ~ +, Extracellular K ~ + exchange rate in proportion, its uneven distribution in the kidney, the study of the distribution of the enzyme in the renal tubular Na ~ + to understand the function of renal reabsorption and pathological changes have some significance.In this experiment, Mayakara Et al lead citrate method, a slight change, the application of p - nitrophenyl phosphate (P - nitrophenylphosphate Na salt P - NPP sodium salt) as a substrate, the same effect can be obtained with high sensitivity and reliable results.