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目的比较两种不同方式灭活的大肠杆菌(OP50)对秀丽隐杆线虫生长发育的影响,以选择合适的灭活食物菌的方法。方法分别采用热处理(65℃水浴)和钴60照射(2000 Gy)的方法灭活OP50,从秀丽隐杆线虫生长发育的影响、OP50的蛋白组成改变以及琥珀酸脱氢酶活性改变等角度评价热处理组、钴60照射组和对照组间的差异,从而确定最有利于秀丽隐杆线虫生长发育的OP50灭活处理方式。结果钴60照射OP50组饲喂后,秀丽隐杆线虫的生长发育与对照组无差异,而热处理OP50组线虫的发育显著迟缓于对照组。同时噻唑蓝[3-(4,5)-dimethylthiazol(-2-y1)-2,5-diphenyltetrazolium bromide,MTT]检测结果显示,热处理OP50的琥珀酸脱氢酶活性明显低于对照组和钴60照射组。结论钴60照射是秀丽隐杆线虫实验研究中比较合适的OP50灭活方法。钴60照射和热处理OP50对秀丽隐杆线虫生长的差异可能由于OP50细菌中蛋白活性差异所致。本文为排除药物和毒物可能会间接通过影响OP50从而影响秀丽隐杆线虫的发育过程的干扰因素、提高基于秀丽隐杆线虫研究数据的可靠性提供了重要参考。
Objective To compare the effects of two different inactivated Escherichia coli (OP50) on the growth and development of Caenorhabditis elegans, in order to select suitable inactivation of food bacteria. Methods Heat treatment (65 ℃ water bath) and cobalt 60 irradiation (2000 Gy) were used to inactivate OP50 respectively. The effects of heat treatment on the growth and development of C. elegans, the protein composition of OP50 and the change of succinate dehydrogenase activity Group, cobalt 60 irradiation group and control group to determine the OP50 inactivation treatment that is most conducive to the growth and development of C. elegans. Results The growth and development of Caenorhabditis elegans were not different from that of the control group after the cobalt-60 exposure to OP50. However, the development of the nematode in the OP50 group was significantly slower than that of the control group. At the same time, the MTT results showed that the activity of succinate dehydrogenase of OP50 was significantly lower than that of control and cobalt 60 Irradiation group. Conclusion Cobalt 60 irradiation is an appropriate OP50 inactivation method in the experimental study of C. elegans. Differences in the growth of C. elegans between cobalt 60 irradiation and heat treatment of OP50 may be due to differences in protein activity in OP50 bacteria. This article provides an important reference for the exclusion of drugs and poisons indirectly through the influence of OP50 on the development of C. elegans and the improvement of the reliability based on the data of C. elegans.