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目的研究强直性脊柱炎(AS)不同疾病状态外周血Th17细胞比率及其相关细胞因子表达情况,探讨其与AS炎症活动的关系。方法 41例AS患者按Bath强直性脊柱炎活动性分为活动组(n=22)和稳定组(n=19),14例健康人作为对照。采用流式细胞术检测外周血Th17细胞比率,ELISA法检测血浆中的IL-17、IL-1β、IL-6、IL-23及肿瘤坏死因子-α(TNF-α)蛋白水平。结果活动组外周血Th17细胞比率明显高于稳定组及对照组(P<0.05或<0.01)。活动组血浆中IL-17平均浓度以及IL-1β、IL-6、IL-23、TNF-α等表达水平均明显高于稳定组及对照组(P均<0.01);IL-17、IL-1β、IL-6、IL-23、TNF-α之间呈显著相关(P<0.05或<0.01)。结论 Th17及相关细胞因子可能与AS炎症高度活动相关。
Objective To investigate the Th17 cell ratio and the expression of related cytokines in peripheral blood of patients with ankylosing spondylitis (AS) and to explore its relationship with the inflammatory activity of AS. Methods 41 cases of AS patients were divided into active group (n = 22) and stable group (n = 19) according to the activity of Bath ankylosing spondylitis, and 14 healthy people as control. The ratio of Th17 cells in peripheral blood was detected by flow cytometry. The levels of IL-17, IL-1β, IL-6, IL-23 and TNF-α in plasma were detected by ELISA. Results The ratio of Th17 cells in peripheral blood in active group was significantly higher than that in stable group and control group (P <0.05 or <0.01). IL-17, IL-6, IL-23 and TNF-α in the active group were significantly higher than those in the stable group and the control group (P <0.01) 1β, IL-6, IL-23 and TNF-α (P <0.05 or <0.01). Conclusion Th17 and related cytokines may be associated with a high degree of inflammation in AS.