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背景与目的:研究PTEN和P53基因在原发性胆囊癌(HGC)中的表达,以探讨它们在HGC发生发展中的作用,寻找胆囊癌诊断及预后的指标。方法:应用原位杂交(DNA-RNA)技术与免疫组化SP法分别检测64例HGC中PTEN mRNA、PTEN蛋白和P53蛋白的表达。以22例胆囊腺瘤及10例慢性胆囊炎作为对照。结果:①PTEN mRNA和蛋白在HGC组织中的阳性率(56.3%,59.4%)均明显低于胆囊腺瘤组织(81.8%,90.8%)和慢性胆囊炎组织(100%,100%),(P<0.05)。②P53蛋白在HGC组织中的阳性率(62.2%)均明显高于胆囊腺瘤组织(27.2%)和慢性胆囊炎组织(0.0%),(P<0.05)。结论:PTEN缺失和P53突变型蛋白共同参与了HGC的发生发展。
BACKGROUND & AIM: To investigate the expression of PTEN and P53 in primary gallbladder carcinomas (HGC) and to explore the role of PTEN and P53 in the development and progression of HGC and to find out the diagnostic and prognostic indicators of gallbladder carcinomas. Methods: The expressions of PTEN mRNA, PTEN protein and P53 protein in 64 cases of HGC were detected by DNA-RNA in situ hybridization and immunohistochemical SP method. 22 cases of gallbladder adenoma and 10 cases of chronic cholecystitis as a control. Results: ① The positive rates of PTEN mRNA and protein in HGC tissues were significantly lower than those in gallbladder adenomas (81.8%, 90.8%) and chronic cholecystitis tissues (56.3%, 59.4% <0.05). ② The positive rate of P53 protein in HGC tissues (62.2%) was significantly higher than that in gallbladder adenomas (27.2%) and chronic cholecystitis tissues (P <0.05). Conclusion: PTEN deletion and P53 mutant protein participate in the development of HGC.