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本文利用目前对检测凋亡较为敏感的末端去氧核苷酸转移酶介导的原位末端标记法 (TUNEL)结合光镜对海马区进行细胞凋亡检测 ,观察丙泊酚 (异丙酚 )对大鼠脑缺血再灌注后海马区细胞凋亡的影响。 1 材料和方法1.1 动物 SD雄性大鼠 ,体质量 2 80～ 32 0 g,购于上海西普耳—必凯
In this study, we detected the apoptosis of hippocampus by using terminal deoxynucleotidyl transferase-mediated end-labeling (TUNEL) combined with light microscopy to detect the apoptosis in hippocampus. We observed the effects of propofol (propofol) On Apoptosis of Hippocampus Region Following Cerebral Ischemia and Reperfusion in Rats. 1 Materials and methods 1.1 Animals SD male rats, body weight 2 80 ~ 32 0 g, was purchased from Shanghai Xi Puer - will Kay