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Micro RNA(mi RNA)是一类非编码单链小分子RNA,广泛参与人类各种生理、病理过程。最新研究提示,mi RNA在子宫肌瘤中起着重要调控作用,该研究试图对子宫肌瘤组织中差异表达mi RNAs进行表达验证及靶基因鉴定,结果发现,与瘤旁组织相比,mi R-363、mi R-490、mi R-135b等的表达水平在肌瘤组织中有着4~6倍的上调,而mi R-217、mi R-590、mi R-451则下调3~5倍。通过软件预测结合表达定量分析,发现其中mi R-363的靶基因为卵泡激素相互作用蛋白1基因(folliculin interacting protein 1,FNIP1)和溶质载体家族蛋白12成员5(solute carrier family 12 member5,SLC12A5)。mi R-135b的靶基因核受体亚科3 C组,成员2(nuclear receptor subfamily 3,group C,member 2,NR3C2)在肌瘤组织中表达有显著下降,而mi R-590的靶基因锌指蛋白367基因(zinc fi nger protein 367,ZFN367)和去泛素水解酶1基因(yeast OTU deubiquinating enzyme 1 homolog,YOD1)在肌瘤组织中的表达水平显著上升。进一步的报告基因分析发现,其中FNIP1、NR3C2、ZNF367的3′UTR能够与相应的mi RNA结合。分析相同肌瘤样品中表达水平的关系发现,这三个靶基因表达均与相应的mi RNA呈显著的负相关。该研究的发现为子宫肌瘤的分子机制研究和诊治提供了新的参考。
Micro RNA (mi RNA) is a kind of non-coding single-stranded small RNA, which is widely involved in various physiological and pathological processes in humans. Recent studies suggest that mi RNA plays an important regulatory role in uterine fibroids. This study attempts to verify the miRNAs differentially expressed in uterine fibroids and identify the target genes. The results showed that compared with tumor-adjacent tissues, mi R MiR-363, mi R-490, mi R-135b, etc. have a 4-6-fold up-regulation in myomas and mi R-217, mi R-590 and mi R-451 down 3-5 fold . The results of software prediction of binding expression showed that mi R-363 target genes were folliculin interacting protein 1 (FNIP1) and solute carrier family 12 member 5 (SLC12A5) . mi R-135b target gene nuclear receptor subfamily 3 C group, member 2 (nuclear receptor subfamily 3, group C, member 2, NR3C2) in myoma tissue expression decreased significantly, and mi R-590 target gene The expression of zinc fi nger protein 367 (ZFN367) and yeast OTU deubiquinating enzyme 1 homolog (YOD1) in fibroid tissues increased significantly. Further reporter gene analysis revealed that the 3’UTRs of FNIP1, NR3C2 and ZNF367 could bind to the corresponding mi RNAs. Analyzing the relationship between the expression levels in the same fibroid samples revealed a significant negative correlation between the expression of these three target genes and the corresponding mi RNA. The discovery of this study provides a new reference for the molecular mechanism research and diagnosis and treatment of uterine fibroids.