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目的监测高温与细菌脂多糖复合应激大鼠生命体征与动脉血气等指标的动态变化特点。方法雄性SPF级Wistar大鼠随机分为:常温生理盐水组(C组)、高温生理盐水组(H组)、常温细菌脂多糖组(L组)、高温细菌脂多糖组(HL组)。持续监测动物肛温(Tr)、心率(HR)、平均动脉压(MAP)以及动脉血气的动态变化。结果(1)HL组Tr上升到43.04℃±0.11℃,HR加快到(660±42)次min,MAP下降到(49.0±3.5)mmHg。HL组Tr、HR、MAP与L组[分别为35.00℃±0.21℃、(408±27)次min、(97.2±2.3)mmHg]的差异有统计学意义(P<0.01),HL组HR、MAP与H组[分别为(576±33)次min、(67.2±4.8)mmHg]的差异亦有统计学意义(P<0.01)。(2)HL组PaO2、HCO-3、PaCO2于致伤40min时即出现明显下降,致伤120min时分别为(11.59±1.11)kPa、(10.42±1.06)mmolL、(2.82±0.81)kPa。结论高温与细菌内毒素复合应激能够促发与加重生命体征、动脉血气的改变以及全身炎症反应综合征。
Objective To monitor the dynamic changes of vital signs and arterial blood gases in rats induced by high temperature and bacterial lipopolysaccharide (LPS). Methods Male SPF Wistar rats were randomly divided into normal saline group (group C), high temperature saline group (group H), normal temperature lipopolysaccharide group (group L) and high temperature bacterial lipopolysaccharide group (HL group). Animal rectal temperature (Tr), heart rate (HR), mean arterial pressure (MAP) and arterial blood gas dynamics were continuously monitored. Results (1) Tr increased to 43.04 ℃ ± 0.11 ℃ in HL group, HR increased to (660 ± 42) min, MAP decreased to (49.0 ± 3.5) mmHg. There were significant differences in HL, Tr, HR, MAP and L group (35.00 ℃ ± 0.21 ℃, (408 ± 27) min, (97.2 ± 2.3) mmHg] The differences between MAP and H group [(576 ± 33) min, (67.2 ± 4.8) mmHg, respectively] were also statistically significant (P <0.01). (2) PaO2, HCO-3 and PaCO2 in HL group decreased significantly after injury for 40min, and were (11.59 ± 1.11) kPa, (10.42 ± 1.06) mmolL and (2.82 ± 0.81) kPa at 120min. Conclusion High temperature and bacterial endotoxin stress can promote and aggravate vital signs, arterial blood gas changes and systemic inflammatory response syndrome.