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目的 :通过 76例肺癌切除标本进行免疫组化染色来探索癌相关基因 c- erb B- 2、ras p2 1和 p5 3与肺癌的组织分型和 TNM分期之间的关系。方法 :随机选取 76例肺癌手术切除标本 ,其中鳞癌 35例 ,腺癌 2 2例 ,小细胞癌10例 ,大细胞癌 4例 ,腺鳞癌 5例。TNM 期 8例 , 期 35例 , 期 33例。均按文献方法进行免疫组化染色后按阳性表达率进行统计分析。结果 :c- erb B- 2和 p5 3的总阳性表达率分别为 6 4.5 %及 5 2 .6 % ,且在小细胞癌中分别高达 10 0 %和 90 .0 % ,明显超过 ras p2 1(P<0 .0 1) ,但 ras p2 1在腺癌中的表达率 (72 .3% )高于 c- erb B- 2和 p5 3。同时 ,c- erb B- 2在 TNM 期中 87.9%的表达率也高于 、 期和 ras p2 1、p5 3在 期的表达率 (P<0 .0 5 )。结论 :c-erb B- 2过度表达与预后差 ,恶性度高 ,病程长或晚期肺癌有关联 ;ras p2 1可能参与腺癌的发生 ;p5 3则可能与恶性度高 ,预后差的小细胞癌的发生和转归有关。
OBJECTIVE: To investigate the relationship between the expression of c-erb B-2, ras p21 and p53 in lung cancer tissues and TNM staging by immunohistochemical staining of 76 cases of lung cancer resected specimens. Methods: Totally 76 cases of resected lung cancer were selected randomly, including 35 cases of squamous cell carcinoma, 22 cases of adenocarcinoma, 10 cases of small cell carcinoma, 4 cases of large cell carcinoma and 5 cases of adenosquamous carcinoma. 8 cases of TNM, 35 cases, 33 cases. According to the method of literature, immunohistochemical staining was used for statistical analysis according to the positive expression rate. RESULTS: The total positive rates of c-erb B-2 and p5 3 were 66.5% and 52.2%, respectively, and were as high as 100% and 90.0% in small cell carcinoma, respectively, which were significantly higher than those in ras p21 (P <0.01), but the expression rate of ras p21 in adenocarcinoma (72.3%) was higher than that in c-erb B- 2 and p5-3. Meanwhile, the expression rate of 87.9% of c-erb B-2 in TNM stage was also higher than that in stage and ras p21, p5 3 (P <0.05). CONCLUSIONS: The overexpression of c-erb B-2 is associated with poor prognosis, high malignancy, long duration of disease or advanced lung cancer; ras p21 may be involved in the development of adenocarcinoma; p5 3 may be associated with malignant and poor prognosis of small cells The occurrence of cancer and the outcome.