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目的建立同时测定十味活血丸中升麻苷和甘草苷含量的HPLC方法。方法采用HPLC法,色谱柱为D iamonsil C18(200 mm×4.6 mm,5μm),流动相为乙腈-体积分数为0.5%的冰醋酸溶液(体积比为15∶85),流速:1.0 mL.m in-1,检测波长:276 nm,柱温:40℃。结果十味活血丸中升麻苷和甘草苷与其他成分分离良好。升麻苷的质量浓度在2.86~28.6 mg.L-1(r=0.999 7)、甘草苷的质量浓度在0.774~7.74 mg.L-1(r=0.999 9)内与峰面积呈良好的线性关系,升麻苷和甘草苷的平均回收率分别为99.2%和99.0%,RSD(n=9)分别为1.8%和1.6%。结论该法可用于十味活血丸中升麻苷和甘草苷的含量测定。
Objective To establish a HPLC method for simultaneous determination of the content of irinoside and glycyrrhizin in Shiwei Huoxue Pills. Methods HPLC method was used. The chromatographic column was Diamonsil C18 (200 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.5% volume glacial acetic acid solution (15:85 volume ratio). The flow rate was 1.0 mL.m. In-1, detection wavelength: 276 nm, column temperature: 40°C. RESULTS Sepsis and glycyrrhizin were well separated from other ingredients in Shiwei Huoxue Pills. The mass concentration of colicin was 2.86-28.6 mg.L-1 (r=0.999 7), and the mass concentration of glycyrrhizin was 0.774-7.74 mg.L-1 (r=0.999 9). Relationships, the average recoveries of ricinin and glycyrrhizin were 99.2% and 99.0%, respectively, and RSD (n=9) were 1.8% and 1.6%, respectively. Conclusion The method can be used for the determination of the content of iridine and glycyrrhizin in Shiwei Huoxue Pills.