垃圾填埋场渗滤液中古细菌群落16S rRNA基因的ARDRA分析

来源 :生态学报 | 被引量 : 41次 | 上传用户：miaohaikun0

【摘要】

：

利用特异性的引物对 ,选择性扩增垃圾填埋场渗滤液中古细菌群落的 1 6 S r RNA基因片断 ,在此基础上建立 1 6 Sr DNA克隆文库。经古细菌通用寡核苷酸探针的原位杂交筛选后 ,克隆文库内古细菌 1 6 S r DNA扩增片断的多样性通过ARDRA分析 ( amplified r DNA restriction analysis)而获得。利用 PCR将各重组克隆子内的 1 6 S r D

【作者】

：

黄立南周惠陈月琴罗硕蓝崇钰屈良鹄

【机构】

：

中山大学生命科学学院,中山大学环境科学系

【出处】

：

生态学报

【发表日期】

：

2002年07期

【关键词】

：

垃圾渗滤液古细菌 16S rRNA基因 ARDRA

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