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目的 :探讨高温致神经管畸形 (NTD)的机制。方法 :利用高温致神经管畸形动物模型 ,在神经管发育的不同阶段 ,用免疫组织化学法检测神经生长因子 (nervegrowthfactor,NGF)和NGF受体 (trkA)在神经上皮、基底膜、脊索和神经管周围间充质中的分布状况 ,并观察高温对NGF及其受体的影响。结果 :NGF及其受体广泛分布于神经管及其周围组织 ,并随胎龄增加而呈规律性变化。高温处理后的胚胎神经上皮及其周围间充质中NGF及其受体的免疫组化着色强度明显减弱 ,着色细胞数明显减少。结论 :NGF及其受体的减少 ,可能是高温致NTD机制中的重要环节
Objective: To investigate the mechanism of hyperthermia induced neural tube defects (NTD). Methods: The animal model of neural tube defects caused by hyperthermia was used to detect the expression of nerve growth factor (NGF) and trkA (NGF receptor) in the neural epithelium, basement membrane, chordate and nerves by immunohistochemistry in different stages of neural tube development. The distribution of mesenchyme around the tube and the effect of high temperature on NGF and its receptor were observed. Results: NGF and its receptors were widely distributed in the neural tube and its surrounding tissues, and showed regular changes with increasing gestational age. High temperature treatment of embryonic neural epithelium and its surrounding mesenchyme NGF and its receptor immunohistochemical staining intensity was significantly weakened, significantly reduced the number of colored cells. Conclusion: The decrease of NGF and its receptors may be an important part of NTD mechanism induced by hyperthermia