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为了研究间隙连接在肿瘤促进中的可能作用,本室新近发展了一种水晶体离体培养技术,我们利用这种技术进行了促癌剂TPA促进水晶体过度生长的实验研究,即把20期的鸡胚水晶体培养于含有80ng/ml TPA的DMEM中,39℃下培养24小时。实验结果指出,实验组的水晶体非常明显地大于对照组(P<0.01)。已报道这个浓度的TPA可使间隙连接通讯安全抑制,表明以上结果可能与细胞间通讯的被抑制密切相关。联系到我们过去利用单克隆抗体所作的实验研究,本文结果进一步证明了间隙连接在生长控制中起重要作用;间隙连接通讯的抑制可能正是肿瘤促进的一种细胞机制。此外,本文报道的鸡胚水晶体离体培养似乎也可作为检测有害物质对生长发育影响的实验系统。
In order to study the possible role of gap junctions in tumor progression, we have recently developed an in vitro culture technique of water crystals. We have used this technique to perform an experimental study on the promotion of overgrowth of crystals by TPA, a carcinogen promoter, that is, a 20-period chicken. Embryos were cultured in DMEM containing 80 ng/ml TPA and incubated at 39°C for 24 hours. The experimental results indicate that the experimental crystals of water crystals are significantly larger than the control group (P<0.01). It has been reported that this concentration of TPA can safely inhibit gap junction communication, indicating that the above results may be closely related to the inhibition of cell-cell communication. In connection with our experimental studies using monoclonal antibodies in the past, our results further demonstrate that gap junctions play an important role in growth control; inhibition of gap junctional communication may be a cellular mechanism for tumor progression. In addition, the in vitro culture of chick embryos reported in this paper seems to be an experimental system for detecting the effects of harmful substances on growth and development.