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为应用与推广金鱼草优良品种,以茎尖为外植体进行组织培养。将其组织放在附加BA5.0—10.0mg/l,NAA0.5mg/l,GA_3 1.0mg/l成分的MS培养基上,不定芽的增殖效果最好;在附加NAA0.5mg/l,IAA0.1—0.5mg/l成分的1/2MS培养基上生根效果最好;在MS+BA10mg/l+NAA0.5mg/l+蔗糖3％的培养基上生长缓慢,有利于室温条件下进行种质保存;蔗糖浓度由3％提高到5％,可以比较明显地延缓试管苗退化。
In order to apply and promote the excellent varieties of snapdragon, shoots were used as explants for tissue culture. On the MS medium with BA5.0-10.0mg / l, NAA0.5mg / l and GA_3 1.0mg / l, the best adventitious buds proliferated on the MS medium supplemented with NAA0.5mg / l, IAA0 The best rooting effect was obtained on 1 / 2MS medium with 1-0.5mg / l composition; slow growth on MS + BA10mg / l + NAA0.5mg / l + sucrose 3% medium was conducive to germplasm at room temperature Save; sucrose concentration increased from 3% to 5%, can significantly delay the degeneration of in vitro plantlets.