论文部分内容阅读
用Ｃ－Ｓｒｃ基因产物（ＰＰ６０ｃ－ｓｒｃ）的特异性单克隆抗体Ｍａｂ３２７对１２例胎儿大肠粘膜、１８例癌旁正常上皮、１８例癌旁增生上皮及３７例大肠癌进行免疫组化研究，其阳性率分别为１００．０％（１２／１２），０％（０／１８），６１．１％（１１／１８），５６．８％（２１／３７）。几者间阳性率有显著性差异（Ｐ＜０．００５），其表达量也有显著性差异（Ｐ＜０．０５）。不同分化程度（高、中、低）的管状腺癌ＰＰ６０ｃ－ｓｒｃ阳性率分别为１００．０％（８／８）、６６．７％（１０／１５）、３７．５％（３／８），分化程度越高，ＰＰ６０ｃ－ｓｒｃ阳性率越高（Ｐ＜０．０５），表达量越高（Ｐ＜０．０１）。３例乳头状腺癌中２例有ＰＰ６０ｃ－ｓｒｃ低表达，３例粘液腺癌未检出ＰＰ６０ｃ－ｓｒｃ。结果提示，ＰＰ６０ｃ－ｓｒｃ激活表达与大肠癌发生发展有关，可能是大肠癌变的早期改变，在其癌变过程中ＰＰ６０ｃ－ｓｒｃ表达量升高，并与大肠癌分化有关。
Mab327, a monoclonal antibody specific for the C-Src gene product (PP60c-src), was used to perform immunohistochemical studies on 12 cases of fetal colorectal mucosa, 18 cases of adjacent normal epithelium, 18 cases of paracancerous hyperplasia, and 37 cases of colorectal cancer. The positive rates were 100.0% (12/12), 0% (0/18), 61.1% (11/18), and 56.8% (21/37), respectively. There was a significant difference in the positive rate among several individuals (P<0.005), and there was a significant difference in their expression levels (P<0.05). The positive rates of PP60c-src in tubular adenocarcinomas with different degrees of differentiation (high, medium, and low) were 100.0% (8/8), 66.7% (10/15), and 37.5% (3/8), respectively. The higher the degree of differentiation, the higher the positive rate of PP60c-src (P<0.05) and the higher the expression level (P<0.01). Two of 3 papillary adenocarcinomas had low expression of PP60c-src, and 3 of them had no detectable PP60c-src. The results suggest that the expression of PP60c-src is related to the occurrence and development of colorectal cancer. It may be an early change of colorectal cancer. The expression of PP60c-src is increased in the process of canceration and is related to the differentiation of colorectal cancer.