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目的研究系统性红斑狼疮(SLE)患者外周血中B淋巴细胞亚群在疾病不同分期的分布变化及其临床意义。方法根据SLEDAI积分将36例SLE患者分为SLE活动组和SLE非活动组,选取20例健康体检者作为正常对照组。应用流式细胞术检测研究对象外周血中B淋巴细胞亚群的分布,并与患者的相关指标进行比较分析。结果与正常对照组相比,SLE活动组和非活动组的静止B细胞百分比明显降低,而浆母细胞百分比明显升高;SLE非活动组的记忆B细胞百分比高于正常对照,浆母细胞百分比显著低于SLE活动组,差异均有统计学意义(P<0.05)。SLE患者的浆母细胞百分比与SLEDAI呈正相关(r=0.701,P<0.01)。SLE患者中,抗ds DNA阳性组的浆母细胞百分比较抗ds DNA阴性组明显升高,差异有统计学意义(P<0.05)。结论 B淋巴细胞亚群在SLE患者体内分布存在异常,浆母细胞百分比与疾病活动性有关,B淋巴细胞亚群在SLE的疾病进展中可能发挥着重要作用,可以作为判断疾病活动性的指标。
Objective To study the distribution and clinical significance of B lymphocyte subsets in different stages of the disease in patients with systemic lupus erythematosus (SLE). Methods According to SLEDAI score, 36 patients with SLE were divided into SLE activity group and SLE inactive group, and 20 healthy subjects were selected as normal control group. Flow cytometry was used to detect the distribution of B lymphocyte subsets in the peripheral blood of the study subjects and compared with the related indicators of the patients. Results Compared with the normal control group, the percentage of resting B cells in SLE group and in inactive group was significantly decreased, while the percentage of plasmablasts was significantly increased. The percentage of memory B cells in inactive SLE group was higher than that in normal control group. The percentage of plasmablasts Significantly lower than the SLE activity group, the difference was statistically significant (P <0.05). The percentage of plasmablasts in patients with SLE was positively correlated with SLEDAI (r = 0.701, P <0.01). In SLE patients, the percentage of plasmablasts in anti-dsDNA positive group was significantly higher than that in anti-ds DNA negative group, with statistical significance (P <0.05). Conclusion The distribution of B lymphocyte subsets in SLE patients is abnormal. The percentage of plasmablasts is related to disease activity. B lymphocyte subsets may play an important role in the progression of SLE and may be used as an index to judge the disease activity.