结合和催化是蛋白质重要的生物化学功能,蛋白质结构特异性识别其他分子是其功能的关键。结合位点和催化位点氨基酸是蛋白质行使功能关键位置上的结构体现。在复杂的蛋白质结构和功能研究中,位点氨基酸的组成、使用率等对挖掘分子结构功能关系具有重要作用。位点氨基酸层次的研究将会促进蛋白质与配体相互关系、蛋白质折叠机制和蛋白质设计的研究。“,”Binding and catalysis are two major examples of biochemical functions of proteins, and specific recognition of other molecules is central of protein function. Amino acids located in binding sites and catalytic sites are structural expression when proteins functions in active positions. In the complex study of protein structure and function, it plays an important role for mining amino acid composition, usage, etc. Studies on amino acids can throw light on the study of the interaction of protein and ligand, the relationship between protein structure and function and the design of novel proteins.