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本文根据抗菌肽Polyphemusin I和Spinigerin α的氨基酸序列,结合Pichia pastoris；偏爱密码子,人工设计合成杂合抗菌肽Py-Sa基因。按照正确的阅读框架将其定向克隆至真核表达载体pPICZα A上。经PCR及双酶切鉴定,所转化的宿主细胞中含有插入Py-Sa的重组质粒pPICZαA-Py-Sa,结果成功构建了杂合抗菌肽Py-Sα的真核表达载体。“,”According to the sequences of amino acid about PolyphemusinⅠand Spinigerin α antimicrobial peptides, combined with the partiality codon of Pichia pastoris, design and synthesis hybrid antimicrobial peptide of Py-Sa gene. In the correct reading frame which was cloned into the eukaryotic expression vector pPICZα A. After PCR and the double enzyme digestion to identify the transformation of Host cell containing the Py-Sa recombinant plasmid pPICZαA-Py-Sa. The results revealed the successful construction of hybrid antimicrobial peptide Py-Sa eukaryotic expression vector.