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为探究蜂胶中4种黄酮成分(高良姜素、短叶松素、松属素、柯因)对胰岛素抵抗的改善作用。通过高浓度胰岛素诱导的方式建立胰岛素抵抗HepG2细胞模型;设立正常组、模型组、高良姜素处理组、短叶松素处理组、松属素处理组、柯因处理组,测定各试验组对HepG2细胞增殖、葡萄糖消耗量、糖原含量、已糖激酶(hexokinase,HK)和丙酮酸激酶(pyruvate kinase,PK)的影响。结果显示:4种黄酮成分在有效浓度范围内对胰岛素抵抗HepG2细胞增殖均无显著影响(P>0.05);柯因、短叶松素作用效果不明显(P>0.05);高良姜素和松属素均能不同程度地提高IR-HepG2细胞葡萄糖消耗量(glucose consumption,GC)、肝糖原含量、HK和PK活力(P<0.05)。上述结果表明蜂胶中高良姜素和松属素能较好地调节IR-HepG2糖代谢,改善胰岛素抵抗,为蜂胶产品的深度开发提供参考。
In order to explore the improvement of insulin resistance of four flavonoids in propolis (galangin, short threadyin, metoprolol and kevin). HepG2 cells were induced by high concentration of insulin. HepG2 cells were cultured in normal group, model group, galangin treatment group, shortening treated group, metoprolol treatment group and kevin-treated group. Proliferation, glucose consumption, glycogen content, hexokinase (HK) and pyruvate kinase (PK). The results showed that the four flavonoids had no significant effect on the proliferation of HepG2 cells in an effective concentration range (P> 0.05), while the effect of Keine and Phlebitis was insignificant (P> 0.05) The glucose consumption (GC), hepatic glycogen content, HK and PK activity in IR-HepG2 cells were increased to some extent (P <0.05). These results show that galangin and propolis in propolis can better regulate IR-HepG2 glucose metabolism and improve insulin resistance, providing a reference for the in-depth development of propolis products.