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目的：探讨同种异体骨移植免疫排异反应与移植物骨诱导活性内在相互作用．方法：新鲜骨、自消化抗原去除骨、骨基质明胶、脱钙骨基质分别植入小鼠肌肉，行免疫学、组织学和碱性磷酸酶检测．结果：４种同种异体骨移植物均诱发免疫排异，以新鲜骨最强烈，骨基质明胶最弱．自消化抗原去除骨、骨基质明胶和脱钙骨基质抑制体外淋巴细胞增殖，以骨基质明胶的抑制作用最显著．自消化抗原去除骨、骨基质明胶和脱钙骨基质均在体内诱导异位成骨，骨基质明胶的骨诱导活性最佳．结论：同种异体骨移植诱发的免疫排异反应与其骨诱导活性呈负相关
Objective: To investigate the intrinsic interaction between allograft immune rejection and graft osteoinductive activity. Methods: Fresh bone, self-digested antigen-removed bone, bone matrix gelatin, and decalcified bone matrix were implanted into the muscle of mice, respectively. Immunohistochemistry, histological and alkaline phosphatase assays were performed. Results: Allograft bone allografts induced immunological rejection, with the strongest fresh bone and the weakest bone matrix gelatin. Self-digestion antigen removal of bone, bone matrix gelatin and decalcified bone matrix inhibition of in vitro lymphocyte proliferation, the most significant inhibition of bone matrix gelatin. Self-digested antigen removal of bone, bone matrix gelatin and decalcified bone matrix were induced in vivo ectopic osteogenesis, bone matrix gelatin best osteoinductive activity. Conclusion: The immune rejection induced by allogeneic bone transplantation is negatively correlated with the osteoinductive activity