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目的 探讨早期缺血再灌流损伤心肌组织热休克蛋白 70 (HSP70 )的变化 ,为心源性猝死的法医病理学鉴定寻找新的依据。 方法 建立家兔早期心肌缺血再灌流动物模型 ,用免疫组化SABC法观察HSP70的表达。 结果 心肌缺血 15min再灌流 30min后 ,再灌流区心内膜下可见HSP70阳性表达 ;缺血30min再灌流 30min后 ,HSP70阳性反应细胞数目较多 ,散布于心肌全层 ;缺血 6 0min再灌流 30min后 ,再灌流区阳性表达明显减少 ,而其边缘见阳性表达略有增强。各实验组正常区和对照组心肌组织均未见阳性反应。 结论 心肌组织HSP70的阳性表达是一项显示心肌早期缺血再灌流损伤的灵敏指标 ,HSP70的免疫组化检测对缺血再灌流损伤所致心源性猝死的死后诊断具有一定的应用价值。
Objective To investigate the changes of heat shock protein 70 (HSP70) in myocardial tissue during early ischemic and reperfusion injury, and to find a new basis for forensic pathological identification of sudden cardiac death. Methods The animal model of early myocardial ischemia-reperfusion in rabbits was established. The expression of HSP70 was detected by immunohistochemical SABC method. Results Myocardial ischemia 15min after reperfusion 30min, reperfusion area subendocardial HSP70 positive expression; 30min ischemia 30min reperfusion 30min, the number of HSP70-positive cells scattered throughout the myocardium; ischemic 60min reperfusion After 30 minutes, the positive expression of the reperfusion area was significantly reduced, while the positive expression of the margin was slightly enhanced. There was no positive reaction in the normal and control groups in each experimental group. Conclusion The positive expression of HSP70 in myocardial tissue is a sensitive indicator of early myocardial ischemia-reperfusion injury. The immunohistochemical detection of HSP70 has some value in post-mortem diagnosis of sudden cardiac death caused by ischemia-reperfusion injury.