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目的:证实在不明原因的肝炎患者血清中一种新型病毒(TTV)的存在,分析其感染特征。方法:用地高辛素标记基因探针,斑点杂交法检测未知和已知病原的肝炎患者75例,正常对照17例。结果:92例血清TTVDNA总检出率为23.91%(22/92)。其中非甲～非戊型肝炎病人组检出率为35%(7/20),甲～戊肝组21.81%(12/55),正常对照组17.65%(3/17);急性肝炎中36.84%(7/19),慢性肝炎中21.88%(7/32),重症肝炎中40%(2/5),肝硬化中15.79%(3/19)。结论:TT病毒可能是导致病毒性肝炎的一种新型病原,可以单独感染或与其他病毒重叠感染,亦可有健康携带状态。感染后出现急性慢性甚至重度肝损伤。
Aims: To confirm the presence of a novel virus (TTV) in the serum of patients with unexplained hepatitis and to analyze its infection characteristics. Methods: Digoxigenin-labeled gene probes and dot blot hybridization were used to detect 75 patients with unknown and known pathogenic hepatitis and 17 normal controls. Results: The total detection rate of TTVDNA in 92 cases was 23.91% (22/92). The detection rate was 35% (7/20) in patients with non-hepatitis A and non-hepatitis E, 21.81% (12/55) in patients with hepatitis A and E, 17.65% (3/17) in patients with normal hepatitis and 36.84 % (7/19), 21.88% (7/32) in chronic hepatitis, 40% (2/5) in severe hepatitis and 15.79% (3/19) in cirrhosis. Conclusion: The TT virus may be a new type of pathogen that causes viral hepatitis. It can be infected alone or overlapped with other viruses, and can also be in a healthy state. Acute chronic or severe liver injury occurs after infection.