0引言免疫组化是利用抗原与抗体特异性结合的原理,通过化学反应使标记抗体的显色剂(荧光素、酶、金属离子、同位素)显色来确定细胞内抗原的成分(主要是多肽和蛋白质),对其进行定位、定性及定量的研究~([1])。作者所在实验室,有丰富的实体肿瘤组织(特别是食管癌组织)石蜡切片免疫组化的实验经验。结合现有的相关文献~([2-4]),现将实体肿瘤组织(特别是食管癌组织)石蜡切片免疫组化所需试剂、耗材、设备和具体实 0 Introduction Immunohistochemistry is the use of antigen and antibody-specific binding principle, the chemical reaction of the labeled antibody color reagent (fluorescein, enzyme, metal ions, isotopes) to determine the color of the intracellular antigen components (mainly polypeptide And protein), its localization, qualitative and quantitative research ~ ([1]). The author’s lab has a wealth of experimental experience in immunohistochemistry of paraffin sections of solid tumor tissues (esophageal cancer in particular). Combined with the existing literature ~ ([2-4]), now the solid tumor tissue (especially esophageal cancer) paraffin section immunohistochemical reagents, supplies, equipment and specific