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为了育成园艺上缺乏的黄色山茶花品种,Lian等1986年曾用Camellia Pitardii Var.yunnanica和C.chrysantha(金花茶)的种间杂交种进行过幼胚培养;Nadamiisu等1986年也用了C.Uiet—namensis和C.chrysantha(金花茶)的杂交种进行过子叶培养。试验所用的母体植株是六倍体,父本金花茶是二倍体。在本研究中,亲本(蝶千岛、金花茶)和它们的杂种幼胚培养成的植株全部是二倍体(2n=30),并且通过精确地分析查明了离体培养下的植株恰好是两亲本种间杂种核型。
In order to breed the lack of yellow camellia species in horticulture, Lian et al. (1986) used immature embryo culture with interspecific crosses of Camellia Pitardii Var. Yunnanica and C. chrysantha (Camellia sinensis); Nadamiisu et al. Used C.Uiet -namensis and C. chrysantha (Camellia sinensis) were cotyledon cultured. The maternal plants used in the experiment were hexaploid and the parental Camellia oleifera was diploid. In the present study, all of the plants grown from the parents (Butterflies Thousand Island, Camellia sinensis) and their hybrid immature embryos were all diploid (2n = 30) and by precise analysis it was ascertained that plants grown in vitro were exactly Interspecific hybrid karyotype.