论文部分内容阅读
目的探讨细胞周期调控基因ｃｙｃｌｉｎ和ＣＤＫ的异常与乳腺癌发生的相关性。方法采集２０例乳腺癌外科手术样品，用原位杂交技术分析了ｃｙｃｌｉｎＤ１、Ｄ３、Ｅ、ＣＤＫ２和ＣＤＫ４ｍＲＮＡ的表达。结果在大多数乳腺癌样品中，这些基因有一种或几种出现高表达。ｃｙｃｌｉｎＤ１高表达的样品１７例（８５％），ｃｙｃｌｉｎＤ３有１２例（６０％），ｃｙ－ｃｌｉｎＥ有７例（３５％），ＣＤＫ２有６例（３０％），ＣＫＤ４有１６例（８０％）。ｃｙｃｌｉｎＤ１和ＣＤＫ４显色最强，其次是ｃｙｃｌｉｎＤ３，ｃｙ－ｃｌｉｎＥ和ＣＤＫ２显色较弱，正常乳腺组织为阴性显色。结论推测ｃｙｃｌｉｎ和ＣＤＫ基因，特别是ｃｙｃｌｉｎＤ１和ＣＤＫ４的异常表达与乳腺癌的发生有密切关系。
Objective To investigate the relationship between the abnormality of cell cycle control genes cyclin and CDK and the occurrence of breast cancer. Methods Twenty breast cancer surgical samples were collected and the expression of cyclinD1, D3, E, CDK2 and CDK4 mRNA was analyzed by in situ hybridization. Results In most breast cancer samples, one or more of these genes showed high expression. CyclinD1 was highly expressed in 17 cases (85%), cyclinD3 in 12 cases (60%), cy-clinE in 7 cases (35%), CDK2 in 6 cases (30%), and CKD4 in 16 cases (80%). The cyclinD1 and CDK4 showed the strongest coloration, followed by cyclinD3. The cy-clinE and CDK2 showed weak coloration. The normal breast tissue showed negative coloration. Conclusion It is speculated that the abnormal expression of cyclin and CDK genes, especially cyclinD1 and CDK4, is closely related to the occurrence of breast cancer.