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选择与SARS病毒相关的寡聚核苷酸片段作为靶分子,利用电喷雾质谱研究人参皂苷与靶分子形成的非共价复合物.探讨了实验条件对单链寡聚核苷酸靶分子与皂苷类化合物相互作用的影响;发现非共价作用强度的大小与人参皂苷苷元、糖链的结构有关,包括人参皂苷苷元的类型、羟基的数目、糖链的长短.发现人参皂苷与单链寡聚核苷酸靶分子相互作用的强度具有如下规律:三醇型苷元>二醇型苷元,三醇型皂苷>二醇型皂苷;在三醇型皂苷中,三糖>二糖>四糖,一糖>苷元,具有相同糖单位的二醇型皂苷的四糖苷中,分子量小的皂苷>分子量大的皂苷,同分异构体中,同为木糖末端的Rb2>Rc,即吡喃型>呋喃型.上述研究结果将有助于阐述人参皂苷的药理活性、构效关系.
Oligonucleotide fragments related to SARS virus were selected as target molecules. Electrospray ionization mass spectrometry was used to study the non-covalent complex formed between ginsenoside and target molecule. The experimental conditions were discussed for single-stranded oligonucleotide target molecules and saponins. The influence of the interaction of the compounds; found that the size of the non-covalent interaction strength is related to the structure of ginsenosides and sugar chains, including the type of ginsenosides, the number of hydroxyl groups, and the length of the sugar chains. Discovery of ginsenosides and single chains The intensity of the interaction of oligonucleotide target molecules has the following rules: triol-type aglycone> diol-type aglycone, triol-type saponin> diol-type saponin; triol-type saponin, trisaccharide> disaccharide> Tetrasaccharides, monosaccharides> Aglycones, tetraglycosides of glycol-type saponins with the same sugar unit, small-molecular-weight saponins> high-molecular-weight saponins, and isomers, both of which are xylose-terminated Rb2>Rc, That is, pyridine> furan. The above results will help explain the pharmacological activity and structure-activity relationship of ginsenosides.