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目的探讨肾移植术后大鼠T细胞亚群动态变化与急性排斥反应的关系。方法 15只健康SD大鼠为正常对照组;15只行假手术SD大鼠为假手术组;16只SD大鼠作为供者、16只SD大鼠作为受者建立同系基因移植为非急性排斥组;16只SD大鼠为受体建立异系基因移植为急性排斥组,供体为16只Wistar大鼠。监测4组大鼠术后第7天T细胞亚群及肾功能变化,并对其组织病理学急性排斥反应严重程度进行半定量评分。结果急性排斥组在术后第7天血清肌酐[(115.35±30.09)μmol/L]、尿素氮[(19.80±10.71)mmol/L]水平和急性排斥反应半定量评分[3(3,3)分]均明显高于正常对照组[(17.80±4.45)μmol/L、(4.97±0.49)mmol/L、0(0,0)分]、假手术组[(47.06±4.28)μmol/L、(5.75±1.06)mmol/L、0(0,0)分]和非急性排斥组[(55.31±8.48)μmol/L、(8.25±1.57)mmol/L、0(0,1)分](P均<0.01);急性排斥组CD4+T细胞比率[(27.98±6.07)%]和CD4+/CD8+比值(2.13±0.66)明显高于非急性排斥组[(8.23±2.63)%、0.20±0.06]、假手术组[(9.84±2.87)%、0.22±0.10]和正常对照组[(8.94±2.44)%、0.20±0.07],CD8+T细胞比率[(13.85±3.74)%]明显低于非急性排斥组[(43.08±6.26)%]、假手术组[(46.67±6.94)%]和正常对照组[(43.69±9.82)%](P均<0.01)。结论 T细胞亚群检测对评价肾移植术后免疫功能状态有一定价值,有助于肾移植术后急性排斥反应的早期诊断和治疗。
Objective To investigate the relationship between the dynamic changes of T cell subsets and acute rejection after renal transplantation in rats. Methods Fifteen healthy SD rats were normal control group. Fifteen sham-operated SD rats were sham-operated group. Sixteen SD rats were used as donors and sixteen SD rats were used as recipients to establish homologous gene transplants as non-acute rejection 16 SD rats were used as recipients to establish allogeneic transplantation group as acute rejection group, donor was 16 Wistar rats. The T cell subsets and renal function were monitored on the 7th postoperative day in the 4 groups, and the severity of acute rejection of histopathology was evaluated semi-quantitatively. Results The levels of serum creatinine [(115.35 ± 30.09) μmol / L] and urea nitrogen [(19.80 ± 10.71) mmol / L] and the acute rejection score [3 Points were significantly higher than those in the normal control group [(17.80 ± 4.45) μmol / L, (4.97 ± 0.49) mmol / L, 0 (0,0) (5.75 ± 1.06) mmol / L, 0 (0,0) and no acute rejection group (55.31 ± 8.48 μmol / L, 8.25 ± 1.57 mmol / L and 0 P <0.01). The ratio of CD4 + T cells in acute rejection group (27.98 ± 6.07%) and CD4 + / CD8 + ratio (2.13 ± 0.66) was significantly higher than that in non-acute rejection group (8.23 ± 2.63) (8.94 ± 2.44)%, 0.20 ± 0.07], and the ratio of CD8 + T cells [(13.85 ± 3.74)%] in the sham operation group were significantly lower than those in the sham operation group (9.84 ± 2.87% vs 0.22 ± 0.10% (43.08 ± 6.26)% in the non-acute rejection group, (46.67 ± 6.94)% in the sham operation group and (43.69 ± 9.82)% in the normal control group (all P <0.01). Conclusion The detection of T cell subsets has some value in evaluating immune function after renal transplantation, which is helpful for the early diagnosis and treatment of acute rejection after renal transplantation.