论文部分内容阅读
采用ＳＰ免疫组化技术，以ＮＤＰＫ／ｎｍ２３Ｈ１特异的单克隆抗体为探针，对人鼻咽癌中ｎｍ２３Ｈ１的表达及其与颈淋巴结转移的关系进行了研究。结果：３１例鼻咽癌中ｎｍ２３Ｈ１基因产物表达的阳性率为４１．９％（１３／３１）。其中无转移组的阳性率为５２．３％（１１／２１），有转移组的阳性率为２０％（２／１０），两组间具有显著性差异（Ｐ＜０．０１），而在转移的颈淋巴结组织中ｎｍ２３Ｈ１产物表达极微或无表达，其阳性率为０，与无转移组比较有显著性差异（Ｐ＜０．０１）。上述结果说明，肿瘤转移抑制基因ｎｍ２３Ｈ１产物的表达，在无转移组呈高表达，在有转移组及淋巴结转移组中呈低表达，其表达与转移呈负相关，说明人鼻咽癌的转移与ｎｍ２３Ｈ１基因的低表达密切相关
The expression of nm23H1 in human nasopharyngeal carcinoma and its relationship with cervical lymph node metastasis were studied by using SP immunohistochemical technique and NDPK / nm23H1 specific monoclonal antibody as probe. Results: The positive rate of nm23H1 gene expression in 31 cases of nasopharyngeal carcinoma was 41.9% (13/31). The positive rate of non-metastasis group was 52.3% (11/21) and the positive rate of metastasis group was 20% (2/10), with significant difference between the two groups (P <0.01) The expression of nm23H1 in metastatic cervical lymph nodes was minimal or no, with a positive rate of 0, which was significantly different from that of the non - metastatic group (P <0.01). The above results show that the expression of tumor suppressor gene nm23H1 is highly expressed in non-metastasis group, low in metastasis group and lymph node metastasis group, and its expression is negatively correlated with metastasis, indicating that metastasis of human nasopharyngeal carcinoma The low expression of nm23H1 gene is closely related