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为揭示欧洲苹果蠹蛾微卫星位点在中国苹果蠹蛾群体遗传学研究中的有效性,以12对微卫星引物对采自中国主要疫区新疆、甘肃、黑龙江的8个苹果蠹蛾地理种群的120头个体在各位点的遗传多样性及扩增稳定性进行研究。所选取的12个微卫星位点中,有8个能够稳定扩增,各位点在大多数种群中均显示偏离哈迪-温伯格平衡,各位点上的平均等位基因数量为3.750～12.500,平均观察杂合度Ho为0.025～0.783,平均期望杂合度He为0.284～0.892。位点Cp2.P和Cp4.56分别具有较低的观察杂合度(0.109、0.025)和较高的近交系数Fis(0.806、0.954),说明这2个位点上的杂合子非常缺乏,其余6个位点均具有较高的杂合度水平和等位基因数量,适用于中国疫区内苹果蠹蛾微卫星分子标记研究。
In order to reveal the validity of European codling moth microsatellite loci in population genetic studies of codling moth in China, 12 pairs of microsatellite primers were collected from 8 dominant populations of codling moth in Xinjiang, Gansu and Heilongjiang provinces, China Of 120 individuals in each locus genetic diversity and amplification stability of the study. Among 12 selected microsatellite loci, 8 of them could stably amplify, and all loci showed deviation from Hardy-Weinberg equilibrium in most populations, and the average number of alleles at each loci was 3.750 ~ 12.500 , The average observed heterozygosity Ho was 0.025 ~ 0.783, the average expected heterozygosity He was 0.284 ~ 0.892. The loci Cp2.P and Cp4.56 had lower observed heterozygosity (0.109,0.025) and higher inbreeding coefficient Fis (0.806,0.954), respectively, indicating that the heterozygotes on these two loci were very scarce and the rest All six loci had higher heterozygosity and allele numbers and were suitable for microsatellite molecular marker study of codling moth in China.