In order to evaluate the influence of the seal structure on door closing force, nonlinear finite element method is introduced to analyze compression deformation of a door seal for SANTANA (name of the car made by Shanghai Volkswagen Co. Ltd). MSC. Marc software is used to analyze the large deformation of the seal and the compression test is done to prove the computational results. The results show that the compression loads of the door seal are larger than the standard value of Shanghai Volkswagen Co. Ltd and the seal structure needs to be optimized. There are consistent relationships between calculating results and experimental results and the simulation method is effective.