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目的:探讨CD24基因在原发性肝细胞癌(HCC)组织中表达的特征及其临床病理意义。方法:应用RT-PCR及免疫组化方法检测CD24基因在42例肝癌组织、相应42例癌旁组织中的表达,分析其与临床病理特征的相关性。结果:RT-PCR显示,CD24mRNA阳性表达与肝细胞癌组织分化程度(P=0.019)及有否肝内转移有关(P=0.031),与肝细胞癌患者性别、HBsAg状态、术前AFP水平、肿瘤是否有包膜、肿瘤大小、癌肿数目无关(P>0.05);Elivision法显示,CD24蛋白阳性表达与肿瘤的组织分化程度(P=0.025)、有无肝内转移密切相关(P=0.008),而与患者性别、HBsAg状态、术前AFP水平、肿瘤是否有包膜、肿瘤大小、癌肿数目无关(P>0.05)。HCC组织中CD24表达较癌旁组织明显升高。结论:HCC组织中CD24基因表达能促进HCC的发生、发展和转移,可作为判断HCC恶性程度、评价预后的指标。
Objective: To investigate the expression of CD24 gene in primary hepatocellular carcinoma (HCC) and its clinicopathological significance. Methods: The expression of CD24 gene in 42 cases of HCC and 42 cases of paracancerous tissues was detected by RT-PCR and immunohistochemistry, and its correlation with clinicopathological features was analyzed. Results: RT-PCR showed that the positive expression of CD24 mRNA was related to the histological grade of hepatocellular carcinoma (P = 0.019) and the intrahepatic metastasis (P = 0.031). The positive correlation between CD24 mRNA and sex, HBsAg status, preoperative AFP, There was no correlation between the number of tumor and the number of tumor (P> 0.05). The Elivision method showed that the positive expression of CD24 protein was closely related to the degree of tumor differentiation (P = 0.025) and the intrahepatic metastasis (P = 0.008 ), But not with gender, HBsAg status, preoperative AFP level, tumor size, tumor size and number of cancer (P> 0.05). HCC tissue CD24 expression was significantly higher than the adjacent tissue. Conclusion: The expression of CD24 in HCC tissue can promote the occurrence, development and metastasis of HCC, which can be used as an index to judge the malignant degree of HCC and evaluate the prognosis.