目的探讨HPLC法在测定感冒退烧片中葛根素含量的应用。方法采用高效液相色谱法测定感冒退烧片中葛根素含量,将测定结果与阴性对照溶液进行比对。结果葛根素在3.9245~392.448μg/ml的范围内呈良好的线性关系(r=0.99998);葛根素的平均加样回收率为100.5%(n=9),RSD=1.1%。结论 HPLC法操作简便、准确、稳定、灵敏度高,适用于感冒退烧片中葛根中葛根素的测定。 Objective To investigate the application of HPLC in the determination of puerarin in cold fever and fever tablets. Methods The content of puerarin in cold fever and fever tablets was determined by high performance liquid chromatography (HPLC), and the results were compared with the negative control solution. Results Puerarin showed a good linearity (r = 0.99998) in the range of 3.9245 ~ 392.448μg / ml. The average recoveries of puerarin were 100.5% (n = 9) and RSD was 1.1%. Conclusion HPLC method is simple, accurate, stable and sensitive. It is suitable for the determination of puerarin in Radix Puerariae.