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目的:探讨IL-15基因修饰的自然杀伤细胞系(简称NK-ustc细胞系)对荷人卵巢癌腹水瘤裸鼠模型的治疗效果。方法:将不同剂量的人卵巢癌细胞注入裸鼠腹腔,观察裸鼠的成瘤时间、成瘤率,确定建立腹水瘤模型的最佳剂量;腹水瘤模型建立后,随机分为治疗组(荷瘤鼠腹腔注射照射后的NK-ustc细胞)和对照组(荷瘤鼠腹腔注射无血清α-MEM培养基),同时设空白对照组(裸鼠腹腔注射照射后的NK-ustc细胞),分别观察各组裸鼠腹围改变及生存时间。结果:腹腔注射2.0×106cells/0.2 m l人卵巢癌HO-8910细胞,裸鼠成瘤时间为(28±5)天,成瘤率为100%;使用照射后的NK-ustc细胞治疗荷腹水瘤裸鼠,治疗组裸鼠的腹围低于对照组,但差异无统计学意义(P>0.05);观察到90天,治疗组中位生存期为54天,对照组裸鼠中位生存期为27天,两组比较,差异有统计学意义(P<0.01),空白对照组全部存活,生长良好。结论:使用NK-ustc细胞治疗卵巢癌腹水瘤裸鼠模型是安全、有效的,为卵巢癌的细胞治疗提供了理论依据。
Objective: To investigate the therapeutic effect of IL-15 gene modified natural killer cell line (NK-ustc cell line) on human ovarian cancer ascites tumor model in nude mice. Methods: Different doses of human ovarian cancer cells were injected into the peritoneal cavity of nude mice to observe the tumorigenic time and tumor formation rate of nude mice, and to determine the best dosage for establishing ascites tumor model. After establishment of ascites tumor model, the mice were randomly divided into treatment group The NK-ustc cells were injected intraperitoneally into the tumor-bearing mice and the control group were injected with serum-free α-MEM into the abdominal cavity of the tumor-bearing mice. At the same time, the blank control group (NK-ustc cells after intraperitoneal injection of nude mice) The abdominal circumference and the survival time of each group were observed. Results: The tumorigenic time of nude mice was (28 ± 5) days with 2.0 × 106cells / 0.2 ml human ovarian cancer HO-8910 cells. The tumorigenic rate was 100%. The irradiated ascites tumor was treated with NK-ustc cells In nude mice, the abdominal circumference of the nude mice in the treatment group was lower than that in the control group, but the difference was not statistically significant (P> 0.05). The median survival time of the control group was 54 days after 90 days. For 27 days, the difference between the two groups was statistically significant (P <0.01), the blank control group all survived and grew well. Conclusion: The use of NK-ustc cells in the treatment of ovarian cancer ascites tumor model in nude mice is safe and effective, providing a theoretical basis for the treatment of ovarian cancer cells.