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目的 探讨神经元型一氧化氮合酶抑制剂 (n NOSI) 7-硝基吲唑 (7- nitroindazole,7-NI)对脑缺血再灌流保护作用的机理。方法 选用 Wistar大鼠 ,制成大脑中动脉闭塞模型(MCAO) ,闭塞 1小时后治疗组给予 7- NI(50 mg/ kg) ,对照组和假手术组给予花生油 5ml/ kg,再灌流 1小时后测定各项指标。结果 再灌流 1小时后 ,7- NI组 NOS(一氧化氮合酶 )活性、MDA(丙二醛 )含量均明显低于单纯缺血再灌流对照组 (P<0 .0 5) ;神经元亚细胞器的异常变化在 7- NI组有明显改善。结论 7- NI对脑缺血再灌流早期有保护作用。
Objective To investigate the protective mechanism of neuronal nitric oxide synthase (nOSI) 7-nitroindazole (7-NI) on cerebral ischemia-reperfusion injury. Methods Wistar rats were used to establish middle cerebral artery occlusion model (MCAO). One hour after the occlusion, the treatment group received 7 - NI (50 mg / kg), the control group and the sham operation group were given peanut oil 5ml / kg and reperfusion for 1 hour After the determination of the indicators. Results NOS (nitric oxide synthase) activity and malondialdehyde (MDA) levels in 7-NI group were significantly lower than those in ischemia-reperfusion group 1 hour after reperfusion (P <0.05). Neurons Aberrant changes in subcellular organelles were significantly improved in the 7-NI group. Conclusion 7-NI has a protective effect in the early stage of cerebral ischemia reperfusion.