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目的研究Smad4、Smad6和Smad7在胰腺癌组织中的改变及其相互关系。方法采用半定量RT-PCR法分别检测了17例胰腺癌和6例正常胰腺组织中Smad4、Smad6和Smad7的mRNA表达情况。结果5例胰腺癌组织不表达Smad4,占29.4%;胰腺癌组织中Smad6和Smad7的表达水平分别为1.15±0.51和1.44±0.84,正常胰腺组织S mad6和Smad7的表达水平分别为0.47±0.18和0.39±0.29,两者相比有统计学意义(P<0.05);Smad6和Smad7的表达水平和Smad4表达无相关性。结论Smad4纯合性缺失在胰腺癌中的发生率约为30%,在胰腺癌的发病机制中占有值得重视的地位;Smad6和Smad7在胰腺癌组织中存在过表达现象,可能是导致胰腺癌发生的原因之一。
Objective To study the changes of Smad4, Smad6 and Smad7 in pancreatic carcinoma and their correlation. Methods The mRNA expressions of Smad4, Smad6 and Smad7 in 17 cases of pancreatic cancer and 6 cases of normal pancreatic tissue were detected by semi-quantitative RT-PCR. Results The expression of Smad4 and Smad7 in 5 pancreatic cancer tissues was 29.4%, while the expression levels of Smad6 and Smad7 in pancreatic cancer tissues were 1.15 ± 0.51 and 1.44 ± 0.84 respectively. The expression levels of S mad6 and Smad7 in normal pancreatic tissues were 0.47 ± 0.18 and 0.39 ± 0.29, respectively (P <0.05). There was no correlation between Smad6 and Smad7 expression and Smad4 expression. Conclusion The incidence of homozygous deletion of Smad4 in pancreatic cancer is about 30%, which plays an important role in the pathogenesis of pancreatic cancer. The over-expression of Smad6 and Smad7 in pancreatic cancer may result in the occurrence of pancreatic cancer One of the reasons.