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对黑龙江省感染玉米弯孢菌叶斑病的叶片进行采集,利用组织分离法对病原菌进行分离,并进一步单孢纯化,经形态学特征并结合真菌核糖体(Internal Transcribed Spacer,ITS)序列分析确认病原菌为新月弯孢菌(Curvu-laria lunata)。细胞壁降解酶和漆酶活性测定显示,病原菌可产生多聚半乳糖醛酸酶(PG)、聚甲基半乳糖醛酸酶(PMG)、纤维素酶(β-1,4-内切葡聚糖酶,Cx)和漆酶(Laccase),PG和PMG的活性在第4天有一个峰值,在第10天PG有一个小峰值;而Cx的活性较低且变化幅度不大。新月弯孢菌生长到72h漆酶酶活达到最高峰,随后酶活逐渐下降;各种酶可连续产生,首先是漆酶酶活迅速增加,其次是PMG,再次是PG,而Cx的变化较小。研究首次发现新月弯孢菌产漆酶,推断其可能也在病菌侵入过程中发挥了重要作用。
The leaves of infected leaves of Curvularia lunata were collected in Heilongjiang Province. The pathogenic bacteria were isolated by tissue isolation method and further purified by single spore. The morphological characteristics and internal sequence cross-linked ITS (ITS) The pathogen is Curvu-laria lunata. The cell wall degrading enzyme and laccase activity assay showed that the pathogen could produce polygalacturonase (PG), polymethylgalacturonase (PMG), cellulase (β-1,4-endo-dextran Carbohydrase, Cx) and Laccase, PG and PMG had a peak at day 4 and a small peak at day 10 PG; whereas Cx was less active and less variable. The laccase activity reached the peak at 72h after the growth of Curvularia lunatus, and then the activity decreased gradually. Various enzymes could be continuously produced. The laccase activity increased rapidly at first, followed by PMG and again at PG, while the change of Cx Smaller. The first study found that Curvularia lunata produced laccase, suggesting that it may also play an important role in the pathogen invasion.