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目的 研究不同条件对愈伤组织诱导、继代和分化的影响 ,探讨白花假龙胆的无性快速繁殖的可能性。方法 用白花假龙胆的胚轴、幼叶及未成熟种子诱导愈伤组织并再生植株。结果 选用MS ,B5和N63种培养基 ,其中以附加 2 ,4 D(2 ,4 二氯苯氧乙酸 ) 3.0mg·L-1+KT(激动素 ) 1.0mg·L-1的MS培养基诱导率最高 ;以附加 6 (6 苄基腺嘌呤 )BA 0 .5mg·L-1+NAA(萘乙酸 ) 0 .2mg·L-1的MS培养基分化苗频率最高 ;以附加 2 ,4 D 2 .0mg·L-1+KT 0 .5mg·L-1的MS培养基愈伤组织的生长最好。结论 外植体、培养基、激素、培养条件等对愈伤组织诱导、继代和分化均有明显影响。
Objective To study the effects of different conditions on the induction, subculture and differentiation of callus and to explore the possibility of asexual rapid propagation of F. gentiana. Methods Calli were induced from the hypocotyls, young leaves and immature seeds of F. gentianae and the plants were regenerated. Results MS, B5 and N63 media were used, with MS medium supplemented with 2,4 D (2,4 dichlorophenoxyacetic acid) 3.0 mg·L-1+KT (Kinetin) 1.0 mg·L-1. The induction rate was the highest; the frequency of MS seedlings was the highest in MS medium supplemented with 6 (6 benzyladenine) BA 0.5 mg·L-1+NAA (naphthaleneacetic acid) 0.2 mg·L-1; with additional 2,4 D The 0.2 mg/L-1+KT 0.5 mg·L-1 MS medium had the best growth. Conclusion Explants, culture medium, hormones, and culture conditions all had significant effects on callus induction, subculture and differentiation.