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目的:观察不同柴胡用量的柴胡水煎液与小柴胡汤对正常小鼠小肠推进功能的影响,并探讨其作用机制。方法:将70只小鼠随机分为七组:正常对照组、小柴胡汤组(小)、小柴胡汤组(中)、小柴胡汤组(大)、及柴胡组(小)、柴胡组(中)、柴胡组(大),分别灌胃生理盐水溶液和相应柴胡用量的小柴胡汤水煎剂,30min后再灌0.4mL的浓度为20mg/mL葡聚糖蓝-2000,20min后处死小鼠,观察各组的小肠推进率。结果:与正常对照组比较,三组小柴胡汤均可增加正常小鼠的小肠色素推进率,与正常对照组比较,差异有统计学意义(P<0.01或P<0.05)。其中,柴胡用量最大的小柴胡汤组(大)与正常对照组、小柴胡汤组(中)、小柴胡汤组(小)相比,增加小肠色素推进率的作用最为明显,与三组比较差异有统计学意义(P<0.01或P<0.05)。与正常对照组比较,不同用量的柴胡水煎液只有柴胡组(大)可增加正常小鼠的小肠色素推进率,与正常对照组比较差异有统计学意义(P<0.01)。除柴胡组(大)外,小柴胡汤组(大)具有明显促进小肠色素推进的作用,差异有统计学意义(P<0.01或P<0.05),但与柴胡组(大)相比无明显的统计学差异(P>0.05)。结论:大剂量的柴胡水煎液与小柴胡汤可以促进正常小鼠小肠推进作用,其作用随柴胡用量的增加而增强。
Objective: To observe the effects of different volumes of Bupleurum decoction and Xiaochaihu decoction on the small intestine prolapse function of normal mice and to explore its mechanism. Methods: Seventy mice were randomly divided into seven groups: normal control group, Xiao Chai Hu Tang group (small), Xiao Chai Hu Tang group (middle), Xiao Chai Hu Tang group (large) and Bupleurum group (Middle), Bupleurum (large) group, respectively, by intragastric administration of saline solution and the corresponding amount of Chaihu decoction decoction, 30min after reperfusion 0.4mL concentration of 20mg / mL dextran blue 2000, Mice were sacrificed after 20 minutes, and the rate of intestinal propulsion in each group was observed. Results: Compared with the normal control group, Xiaochaihu decoction of three groups could increase the intestinal pigment advancing rate in normal mice. The difference was statistically significant (P <0.01 or P <0.05) compared with the normal control group. Among them, Xiaochaihu Decoction (big), the largest amount of Bupleurum, had the most obvious effect on promoting the rate of small intestine pigment compared with normal control group, Xiaochaihu decoction group (middle) and Xiaochaihu decoction group (small) The difference was statistically significant (P <0.01 or P <0.05). Compared with the normal control group, only Bupleurum (Bupleurum) group can increase the rate of small intestine pigment pigment in normal Bupleurum decoction group with different dosage (P <0.01). In addition to Bupleurum (large), Xiao Chai Hu Tang group (large) has a significant role in promoting intestinal pigment, the difference was statistically significant (P <0.01 or P <0.05), but compared with the Bupleurum group No significant statistical difference (P> 0.05). Conclusion: The high dose of Bupleurum decoction and Xiaochaihu decoction can promote the small intestine propulsion in normal mice. The effect of Bupleurum decoction is enhanced with the increase of the dose of Bupleurum.