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在枫树苗期黄萎病几种接种方法中,以浓度为1.8×106个分生孢子/mL的孢子悬浮液浸根接种效果最好,具有接种操作简便、定量、发病快和随机等特点;灌根接种效果最差。土壤接种能反映大田自然发病的优点,接种量为2%(W/W)的麦草培养菌比甜菜籽培养菌接种效果好,直播比移栽的植株接种效果好。结果表明,根据株高、发病率和定植率来评价接种效应是可行的。
Among the several inoculation methods of Verticillium dahliae at the seedling stage of maple, the best effect was obtained by soaking the spores suspension with the concentration of 1.8 × 106 conidia / mL, which was easy to inoculate, quantitative, fast onset and random Characteristics; irrigation root inoculation is the worst. Soil inoculation can reflect the natural disease in the field. The inoculation amount of 2% (W / W) inoculation was better than that in the culture of beet seed, and the inoculation was better than the inoculation. The results showed that it is feasible to evaluate the inoculation effect according to plant height, incidence and colonization rate.