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大量流行病学调查发现,乙肝病毒感染患者胆石症发病率明显增高,近来研究证实,内源性β-葡萄糖醛酸酶(β-G)在胆红素结石发病中起重要作用,我们应用ELISA法测定了32例乙肝病毒慢性携带者及36例健康人血清内源性β-G含量,以探讨乙肝病毒感染与胆红素结石发病的关系。 1 材料和方法 1.1 材料 收集中国医科大学第一临床医院1998-04/1999-04期间32例乙肝病毒感染者(其中无症状乙肝病毒携带者18例,慢性肝炎14例)空腹静脉血4mL,分离出血清4mL-70℃深低温冰箱储存。32例患者中胆囊息肉8例,胃溃疡9例,肠梗阻10例,腹股沟斜疝3例,慢性阑尾炎1例,所有患者均经检
A large number of epidemiological survey found that the incidence of cholelithiasis in patients with hepatitis B virus infection was significantly increased, recent studies have confirmed that endogenous β-glucuronidase (β-G) play an important role in the pathogenesis of bilirubin stones, we use ELISA Method to determine the serum endogenous β-G content in 32 chronic carriers of hepatitis B virus and 36 healthy volunteers to explore the relationship between hepatitis B virus infection and the incidence of bilirubin stones. 1 Materials and Methods 1.1 Materials Collection The first clinical hospital of China Medical University during 1998-04 / 1999-04 32 cases of hepatitis B virus infection (including 18 cases of asymptomatic hepatitis B virus carriers, chronic hepatitis 14) fasting venous blood 4mL, separation Serum 4mL-70 ℃ deep low temperature refrigerator storage. 32 cases of gallbladder polyps in 8 cases, 9 cases of gastric ulcer, intestinal obstruction in 10 cases, 3 cases of inguinal hernia, 1 case of chronic appendicitis, all patients were seized